combine-stains

combine_stains

skimage.color.combine_stains(stains, conv_matrix) [source]

Stain to RGB color space conversion.

Parameters:	stains : array_like The image in stain color space, in a 3-D array of shape (.., .., 3). conv_matrix: ndarray The stain separation matrix as described by G. Landini [R31].
Returns:	out : ndarray The image in RGB format, in a 3-D array of shape (.., .., 3).
Raises:	ValueError If stains is not a 3-D array of shape (.., .., 3).

Notes

Stain combination matrices available in the color module and their respective colorspace:

• rgb_from_hed: Hematoxylin + Eosin + DAB
• rgb_from_hdx: Hematoxylin + DAB
• rgb_from_fgx: Feulgen + Light Green
• rgb_from_bex: Giemsa stain : Methyl Blue + Eosin
• rgb_from_rbd: FastRed + FastBlue + DAB
• rgb_from_gdx: Methyl Green + DAB
• rgb_from_hax: Hematoxylin + AEC
• rgb_from_bro: Blue matrix Anilline Blue + Red matrix Azocarmine + Orange matrix Orange-G
• rgb_from_bpx: Methyl Blue + Ponceau Fuchsin
• rgb_from_ahx: Alcian Blue + Hematoxylin
• rgb_from_hpx: Hematoxylin + PAS

References

[R31]

(1, 2) http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

Examples

>>> from skimage import data
>>> from skimage.color import (separate_stains, combine_stains,
...                            hdx_from_rgb, rgb_from_hdx)
>>> ihc = data.immunohistochemistry()
>>> ihc_hdx = separate_stains(ihc, hdx_from_rgb)
>>> ihc_rgb = combine_stains(ihc_hdx, rgb_from_hdx)